Critical Factors for Successful Real-Time PCR

Critical Factors for Successful Real-Time PCR

Evaluation of a new set of Real-Time PCR for Brucella detection within human and animal samples

A quantitative TaqMn Real-Time PCR assay was developed and its diagnostic value on human serum and livestock samples were evaluated. Brucella species could be distributed through communities as a biological agent. Rapid detection of biological threat agents is critical for timely therapeutic administration. Quantitative real-time PCR provides a rapid, sensitive and specific tool for molecular i...

شناسایی ویروس‌های آنفلوانزای A/H3N2 مقاوم به اسلتامیویر با تست Real-time RT- PCR

Background: Currently, with increasing risk of influenza A virus epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and treatment of these viruses. The H274Y mutation is from the most important drug resistant factors in influenza A viruses. The aim of this study was detection of Oseltamivir...

مقایسه تاثیر مهاری ژل APF و ژل سدیم فلوراید (NAF) بر روی غلظت میکروارگانیزم های پوسیدگی زا در محیط دهان با روش Quantitative Real Time PCR

Background and purpose: Several factors are involved in caries prevention in children. One of the most effective factors is the appropriate use of fluoride. Fluoride induces its main effects in caries prevention through antibacterial effects and topical contact with enamel. In this study the inhibitory effects of sodium fluoride (NaF) and acidulated phosphate fluoride (APF) gels on cariogenic m...

Real-Time PCR: an Appropriate Approach to Confi rm ssDNA Generation from PCR Product in SELEX Process

Background: Aptamers are single stranded DNA (ssDNA) or RNA molecules. The potential of aptamers for binding to the different targets has made them be widely used as the preferred diagnostic and therapeutic tools. DNA aptamers present several advantages over the RNA oligonucleotides due to their higher stability, easier selection, and production. Selection of DNA aptamers which...